Study of Aggregation of Phospholipid Molecules

Authors

  • H. Švecová Department of Analytical Chemistry, Faculty of Science, Palacký University, Olomouc
  • J. Součková Department of Analytical Chemistry, Faculty of Science, Palacký University, Olomouc
  • J. Skopalová Department of Analytical Chemistry, Faculty of Science, Palacký University, Olomouc
  • R. Novotný Laboratory of Microscopy Methods, Faculty of Medicine and Dentistry, Palacký University, Olomouc
  • P. Barták Department of Analytical Chemistry, Faculty of Science, Palacký University, Olomouc

Keywords:

phospholipids, 1,2-distearoyl-sn-glycero-3-phosphatidylcholine, cyclic voltammetry, mercury drop electrode, nephelometry, micelles, liposomes, spontaneous revesiculation

Abstract

The behavior of 1,2-distearoyl-sn-glycero-3-phos­phatidylcholine (DSPC) was studied in spontaneous revesiculation used in preparation of liposomes uncontaminated with residues of organic solvents or surfactants. Cyclic voltammetry at hanging Hg drop electrode, nephelometry and transmission electron microscopy were used to study the aggregation of DSPC. Changes in current and potential of voltammetric charging peaks, similar to those due to formation of micelles in surfactant solutions, were investigated at certain concentrations of phospholipids in neutral phosphate buffer. Simultaneously, changes in turbidity of DSPC solutions were recorded. These concentrations have been characterized as critical aggregation concentrations (0.07–0.10 and 0.19–0.24 mmol l-1).

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Published

2012-03-15

How to Cite

Švecová, H., Součková, J., Skopalová, J., Novotný, R., & Barták, P. (2012). Study of Aggregation of Phospholipid Molecules. Chemické Listy, 106(3), 200–205. Retrieved from http://www-.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/965

Issue

Vol. 106 No. 3 (2012)

Section

Articles

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